Effects of Parenteral Vitamin D3 Supplementation on Hematological Parameters of Healthy Holstein bulls

Vitamin D has important and well-known roles in the regulation and metabolism of calcium and bone health, but in addition to these roles, it has many effects on the body's cells, which are called non-calcium functions of vitamin D. One of the non-calcium effects of vitamin D is its effect on the function and number of blood cells. Twenty healthy bulls were divided into four groups to evaluate the effect of different doses of vitamin D3 on the number of bovine blood cells. Group A received 11,000 units/kg of vitamin D3, group B received 22,000 units/kg of vitamin D3, group C received 33,000 units/kg of vitamin D3, and group D received 44,000 units/kg of vitamin D3. The control group was injected with 10 ml of physiological saline intramuscularly. Blood samples were taken before the injection and 2, 4 and 6 days after the injection and the WBC counts (including granulocytes and lymphocytes), hematocrit, hemoglobin and platelets were examined by a cell counter. The results showed that vitamin D can cause a decrease in white blood cells counts (as well as granulocytes and lymphocytes) and platelet counts and an increase in hematocrit and hemoglobin levels in the blood. Although the increase or decrease mentioned is largely dose- and time-dependent, first, the best group to indicate this is group B, and second, perhaps to study the long-term effects of injections, especially High doses require tests to be performed with larger groups over a longer period