Creation of Mice Expressing Human Antibody by Introduction of a Human Chromosome

A human chromosome or its subchromosomal fragment (SCF) derived from normal fibroblasts was introduced into mouse embryonic stem (ES) cells via microcell-mediated chromosome transfer (MMCT) and viable chimaeric mice were produced from them. Serum antibodies with each human Ig were detected and various V segments were identified in human Ig H, κ and λ transcripts. Upon immunization of the chimaeras with HSA, HSA-specific antibodies with hγ hκ and λ were detected in the sera. A HSA-specific human antibody with hμ producing hybridoma was obtained from fusion of murine myelomas with spleen cells from the chimaeric mouse.