Human ovarian cancer cell lines resistant to cisplatin, doxorubicin, and l-phenylalanine mustard are sensitive to Δ7-prostaglandin Δ1 and Δ12-prostaglandin J2

Abstract The antitumor activity of Δ 7 -prostaglandin A 1 (Δ 7 -PGA 1 ) or Δ 12 -prostaglandin J 2 (Δ 7 -PGJ 2 ) on human ovarian cancer cell lines resistant to cisplatin (CDDP), doxorubicin (ADR), and l-phenylalanine mustard (1-PAM) was studied in vitro . A2780 AD , A2780 (parent cells of A2780 AD ), 2008DDP, and 2008 cells (parent cells of 2008DDP) were used. The antitumor activities of the drugs were defined with 50% inhibitory concentration (IC50) estimated from growth inhibition curves, which were obtained by an indirect colorimetric method. Drug-resistance ratios obtained from IC50 values, by comparing A2780 AD and A2780 cells, were 62.5 for ADR, 4.6 for CDDP, 4.9 for 1-PAM, 1.5 for Δ 7 -PGA 1 , and 1.8 for Δ 7 -PGJ 2 . Those obtained by comparing 2008DDP and 2008 cells were 1.1 for ADR, 16.0 for CDDP, 2.9 for 1-PAM, 2.3 for Δ 7 -PGA 1 , and 3.2 for Δ 7 -PGJ 2 . Thus some human ovarian cancer cells resistant to ADR, CDDP, and 1-PAM remain sensitive to antitumor PGs.