The effects of hypoxia inducible factor - 2α on the proliferation and invasion of the hepatocellular carcinoma cells

Objective To investigate the effects of hypoxia inducible factor- 2α （HIF -2α） on the proliferation and invasion of hepatoeellular carcinoma cell line SMMC -7721 under the anoxia condition. Methods Lipofeetamine - mediated HIF -2α oligodeoxynueleotide （ODN） was transfeeted into SMMC -7721 cells and the expressions of HIF- 2α mRNA and protein were detected to evaluate the transfeetion, The proliferative and adhesive activity was determined by methyl thiazolyl tetrazolium （MTF） method and the invasive activity was determined by a transwell cell culture chamber method. Results cantly by HIF- 2α antisense oligodeoxyribonueleotide （ASODN） （ P The expressions of HIF - 2α mRNA and protein were inhibited signifi- 〈0.01 ）. The blockage of HIF- 2α expression significantly inhibited the adhesive activity （ inhibition ratio 16. 99% - 31.07% ） and invasive activity （ inhibition ratio 17.9% ） （ P 〈 0. 01 ）, with no significant influence on the proliferative activity of SMMC - 7721 cells （ P 〉 0. 05 ）. Conclusion The blockage of HIF - 2α expression inhibits the ad- hesive and invasive activity of SMMC -7721cells, and has no evident impact on their proliferation. Key words: Hypoxia inducible factor - 2α ;  Oligonucleotides ;  hepatocellular carcinoma;  Proliferation ;  Adhesion ;  Invasion