Preparation method and application of stearylglycerol in Lycium ruthenicum Murr

2015 
The invention relates to a preparation method for stearylglycerol in Lycium ruthenicum Murr. The method comprises the following steps: (1) impurity removal with microporous resin: a step of dissolving extract of Lycium ruthenicum Murr with an ethanol solution, separating the obtained solution by using a microporous resin column, carrying out step gradient elution and subjecting eluate to pressure-reduced drying so as to obtain a crude product of stearylglycerol; and (2) silica gel column chromatography separation: a step of subjecting the crude product of stearylglycerol to silica gel column chromatography separation, carrying out elution, collecting fraction, detecting and collecting fraction with an Rf value of 0.1 to 0.2 by using thin-layer chromatography and subjecting the collected fraction to pressure-reduced drying so as to obtain colorless oily 1-[(12E,16E)-12,16-eicosadienoyl]-2-[(E,E)-7,11-octadecadienoyl]-3-stearylglycerol with purity of greater than 95%. The preparation method is simple and has a high recovery rate; and the prepared 1-[(12E,16E)-12,16-eicosadienoyl]-2-[(E,E)-7,11-octadecadienoyl]-3-stearylglycerol has good blood fat-reducing effect.
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