Cloning and sequence analysis of a novel bone morphogenetic protein 4 gene from Cervus nippon

In this paper,a novel gene encoding BMP4 was cloned successfully from total RNA extracted from the liver of Cervus nippon via RT-PCR.Bioinformatics analyses were performed on the nucleotide sequence,protein sequence,conserved functional region as well as hydrophobicity of the target protein.The phylogenetic tree and simulative 3D structure were also constructed for BMP4.The sequence has been submit to Genbank with the accession number HQ877675.The work reported here provides a basis for expression of recombinant Cervus nippon BMP through genetic engineering as well as exploration of its mode of action in the future.