Study on extraction of chenodeoxycholic acid and bilirubin fromgoose gallbladder

Both chenodeoxycholic acid and bilirubin are the main active components of goose gallbladder.The chenodeoxycholic acid and bilirubin were isolated and extracted from the goose gallbladder in the same time through the steps of saponification,neutralization,delamination and so on.The effect of saponification temperature,time and sodium hydroxide concentration were determined through the single factor and orthogonal tests.The results showed that the optimum conditions were as follows:saponification temperature 90℃,1h,sodium hydroxide concentration 8%.Under these conditions,the yield of chenodeoxycholic acid reached 0.9mg/g,the yield of bilirubin reached 0.46mg/g.