Transcriptome analysis of Sitodiplosis mosellana Gehin by RNA-Seq

The wheat midge,Sitodiplosis mosellana,is one of the most important pests in the world.The study aims to get transcriptome of S.mosellana.The transcriptome of S.mosellana was sequenced using an Illumina HiSeq TM 2000 platform.There were 27.88 G datas was obtained which further assembled into 59257 Unigenes with the length of 49861164 bp.Minimum length was 201 bp,maximum length was 29282 bp,average length was 841 bp.Based on the NR,NT,Swiss-Prot,KEGG,GO and KOG databases(Evalue e≤10-10),95029 Unigenes were annotated.In this study,all assembled Unigenes can be broadly divided into biological processes,cellular components and molecular function categories of 50 branches by gene ontology,including metabolic process,binding,catalytic activity and cellular process.Unigenes were further annotated based on KOG category,11279 Unigenes were annotated,which could be grouped into 24 functional categories.In KEGG pathways identification,9110 sequences were annotated,respectively belong to 5 kinds of cellular processes,environmental information process,genetic information process,metabolic and organism system,which can be broadly divided into 32 classes according to the function,including cell growth and apoptosis,cell motility,signal transduction,energy metabolism etc.CDS prediction showed that 30088 sequences can be encoded,which accounted for all the 50.78%.There were 36323 SSR in 59257 Unigenes were,the incidence was 61.30%.The orange wheat blossom midge transcriptome greatly improves our genetic understanding and provides a platform for functional genomics research of this species.