Construction of Co-expressing Plasmids of AgB from Taenia Solium with Porcine CD58 or IFN-γ Gene and Expression in BHK-21 Cells

Cysticercosis is an important zoonosis.The development of efficient,safety and cheap vaccines is one of contemporary major directions.In this study,AgB,CD58 and IFN-γ gene were amplified from the former constructed recombinant plasmids named pGEM-TEasy-AgB,pGEM-TEasy-CD58 and pGEM-TEasy-IFN-γ by PCR and cloned into pBudCE4.1.The acquired recombinant plasmids were transfected into BHK-21 cell with liposome.48 hours after transfection,the expression of AgB,CD58 and IFN-γ were tested by indirect immunofluorescence assay(IFA) or direct immunofluorescence assay.The results showed that AgB,CD58 and IFN-γ were successfully expressed in BHK-21 cells,providing a solid basis for development of DNA vaccine against cysticercosis.