P20-15. Evaluation of HIV-1 subtype B acute envelope-expressing infectious molecular clones

Methods Individual viruses (20) were evaluated for differential tropism in cellular assays (PBMC, monocyte-derived macrophages, PM1, TZM-bl cells, dendritic cells and dendritic cell-T cell trans-infection) and explant tissue assays, (ectocervix, penile glans, rectal and tonsil). Relative replication of each virus among the different models was assessed by infecting with virus for two hours, washing and measuring p24 release in supernatant over time.