Phylogeography of a Specialist Leaf-Mining Weevil, Rhynchaenus dorsoplanatus (Coleoptera: Curculionidae), Associated with Castanopsis Species

ABSTRACT Climatic changes during glacial periods have had a major influence on the recent evolutionary history of living organisms, even in the warm temperate forests, where the land was not covered with ice sheets. We investigated the phylogeographical patterns of the weevil, Rhynchaenus dorsoplanatus (Roelofs) (Coleoptera: Curculionidae), a host-specific leaf miner of Castanopsis (Fagaceae) that grows in the broadleaved evergreen forests of Japan. We examined 2343 bp of mitochondrial DNA (mtDNA) for 171 individuals collected from new infested leaves of Castanopsis at 55 localities in Japan. Spatial analysis of molecular variance showed a significant difference (φCT = 0.14, P < 0.001) in genetic structure of the leaf-mining R. dorsoplanatus between the southwestern and the northeastern areas within the main islands of Japan. We also observed a large gap between the southwestern and northeastern clades (φCT = 0.79, P < 0.001) with respect to the mtDNA sequences of the seed-boring Cuivulio hilgendorfi (Har...