Anti-aging Effect on Skin with Jaeum-Dan (JED)

Objectives: Jaeum-Dan (JED) I and II are new formulas composed with oriental herbs. We investigated the anti-aging effects on skin with water extracts of JED 1 , JED II, JJZD IIJ and each Bupleuri Radix, Lonicerae Caulis, Coicis Semen, Armeniacae Amarum Semen, Plantaginis Herba. Methods: We measured various effects related to skin such as the proliferation of keratinocytes, the inhibition of reactive oxygen species (ROS) generation, 5a-reductase activity, the gap junctional intercellular communication, the amount of melanin and the synthesis of MMP-1 and type I procollagen. Results: The proliferation of keratinocytes were increased in JED I , 11, ID and water extracts of Bupleun Radix, Plantaginis Herba. The production of ROS were decreased in JED I , II, IJl and water extract of Bupleun Radix. 5-reductase activity were decreased in JED I , II, lII and water extract of Lonicerae Caulis. Gap junctional intercellular communcation were increased to normal level in water extract of Coicis Semen. The amount of melanin were decreased in JED I , II, IU and water extract of Armeniacae Amarum Semen, Plantaginis Herba. The synthesis of MMP-1 and type I procollagen were positive in JED I , II, ID and water extract of Plantaginis Herba. Conclusions: The results of our study indicate that each 5 herbs have anti-aging effects and the complex of JED I with rl were best in anti-aging effects on skin.