Boronic Acid matrices for the affinity purification of glycoproteins and enzymes.

: Boronic acids immobilized on insoluble matrices have been used for over a decade to purify proteins and enzymes (1,2). Boronic acid matrices have been used to purify: 1. Glycoproteins, 2. Enzymes for which a boronic acid moiety is an inhibitor, and 3. Enzymes that bind to specific dial-containing cofactors (such as UTP or NADP(+)).