Application of Eletrodialysis to Enrich DNA Online in Microchip

Based on the technology of electrodialysis,a new microchip with membrane separation like "sandwich" was make for the non-damage enrichment of DNA online.First,by using genefinder,a cyanine dye,as fluorescence indicator,the enrichment of DNA was monitored in the microchip.Then λ-DNA and λ-DNA-hemoglobin,as model compounds,were separately used to study the characteristic of capturing and enriching DNA in this chip.Final,gel electrophoresis was done to study if DNA was denaturation and polymerase chain reaction(PCR) was done to prove the concentrate of DNA enriched can be used to the further bio-analysis.The results were as follows: by using genefinder,a cyanine dye,as fluorescence indicator,on-line enrichment of DNA in the microchip were monitored successfully,the chip could be used to capture and enrich DNA scathelessly from single and simple mix system,the concentrate mutiple was accessible to 50,and the recovery efficiency was about 30%,concentration can be used for further biological analysis.