The role of Let-7a in mouse allergic rhinitis

To investigate the role of Let-7a in mouse allergic rhinitis,a total of 24 8-week old mature femaleC57BL/6 mice were randomly divided into four groups:Let-7a group,Let-7a control group,OVA group and PBSgroup,with 8 mice in each group.Mice in Let-7a group,Let-7a control group and OVA group were sensitized withovalbumin(OVA)to construct allergic rhinitis model.Mice in Let-7a group were administrated with Let-7a andliposome2000 mixture half an hour before the first,second and third challenge,and mice in Let-7a control groupreceived Let-7a control and liposome2000.PBS group was given PBS instead of OVA.Nasal rubs,eosinophilic cellinfiltration and goblet cell hyperplasia was estimated and local IL-4,IL-13 and IFN-γ cytokines in the localmucosa were measured.We found that compared with Let-7a control group,Let-7a group had more nasal rubs,eosinophilic infiltration and goblet cell hyperplasia,and increased levels of IL-4 and IL-13 in nasal mucosa(P<0.05),while no significant difference was observed for IFN-γ.So we concluded that Let-7a promotes the occurrenceand development of allergic rhinitis.