Quantitation of 5-fluorouracil incorporation into RNA by high-performance liquid chromatography without the use of radioactive precursors

In this note we report the development of a method for identifying and quantitating 2',3'-FUMP (the 2' and 3' mixed isomers of fluorouridine monophosphate) in alkaline hydrolystates of murine tumor RNA using high-performance liquid chromatography (HPLC) and UV spectrophotometry