Chemical methods in association with physical methods in drug analysis

Abstract Physical methods, namely spectroscopy and separation techniques such as high-performance liquid chromatography, in which the analyte is not chemically changed during analysis, have gained increasing popularity in the field of pharmaceutical analysis. Nevertheless, chemical manipulation in combination with such methods will continue to play a vital role, as will be demonstrated with examples selected from methods developed for two new, cardioactive drugs, nadolol and captopril, and from other recent literature references.