Electrospun Nanofibrous Membrane Biosensor for lactate

A new technique for the detection of lactate using lactate d ehydrogenase (LDH) immobilized in polyvinyl alcohol (PVA) nanofibers is described in this article. A major stability of the enzyme is reached through its immobilization, allowing its use as biosensor in different environmental conditions. This immobilization is attained using a novel but relatively simple technique named electrospinning. There is an increase in the 340 nm absorbance once the immobilized enzyme is introduced in the reaction media. This increase in the absorbance can be used to sense the presence of lactate in different environments. The immobilized LDH system presents different advantages in comparison with traditional methods for the detection of lactate such as an improved sensibility and a better response time.