Determination of sesamin and sesamolin in sesameusing HPLC with C_(18) purification

The content of sesamin and sesamolin in sesame were determined by HPLC,and the conditions of the ultrasonic-assisted extraction of the two substances were studied.By the single factor experiments and orthogonal experiments,the extraction conditions were optimized as follows:n-hexane-MeOH(volume ratio 60∶40) as extraction solvent,ratio of liquid to material 30∶1,extraction temperature 65℃,ultrasonic extraction time 12 min.The C18 adopted in the pre-processing of sample could enhance the purification effect and decrease the inspectional molestation.