Prior exposure to gonadotrophins prevents the subsequent antigonadotrophic actions of cloprostenol by a cyclic AMP-dependent mechanism in cultured human granulosa cells

The antigonadotrophic action of a prostaglandin F 2α analogue, cloprostenol, has been investigated in human granulosa cells obtained from cycles stimulated for in vitro fertilization and induced to secrete luteal quantities of progesterone by culture in serum-supplemented medium