Phytochemical Constituents Analysis and Neuroprotective Effect of Leaves of Gemor (Nothaphoebe Coriacea) on Cadmium-Induced Neurotoxicity in Rats: An In-Vitro Study

The objectives of this study were to determine phytochemical compositions and neuroprotective effects of aqueous extract of leaves of gemor against Cd-induced neurotoxicity in vitro. Neurotoxicity was induced by 3 mg/l of Cd in a form of cadmium sulphate (CdSO4) in brain homogenate. Phytochemical screening of plant extract was determined using the qualitative method to measured the flavonoid, alkaloid, steroid, triterpenoid, and phenolic content. The neurotoxicity effect of Cd and neuroprotective effect of the aqueous extract of leaves of gemor (Nothaphoebe coriacea) was determined by assessing the concentration of hydrogen peroxide (H2O2), malondialdehyde (MDA), carbonyl compound (CC), and the activity of superoxide dismutase (SOD) and catalase (CAT) enzymes. Phytochemical screening results showed that the plant extract contained all phytochemical constituents with phenolic was the highest. Administration of Cd led to a significant elevation of H2O2, MDA, and CC level and significantly decreased the activity of SOD and CAT compared to control. Administration of plant extract showed significant decreases in H2O2, MDA and CC level and significant increase in SOD and CAT activity compared to Cd administration group. The results suggest that the administration of aqueous extract of leaves of gemor provide significant protection against Cd-induced neurotoxicity in vitro. Keywords: Antibiotic, Amylase, Catalase, Kinetic Parameters, Neonatal Sepsis, Saliva