Molecular epidemiology of Vibrio parahemolyticus by pulse field gel electrophoresis.

The purpose of this study is using pulsed field gel electrophoresis(PFGE) and PCR typing to analyze Vibrio parahemolyticus isolated from outbreaks during 2008-2009 and to trace the infection source.Virulence genes tdh and trh were detected by PCR,and PFGE was used for subtyping isolates.Patterns were compared by using BioNumerics.The results showed that within the 38 isolates,31 were tdh positive and 2 were trh positive.The 38 strains were clustered into 24 PFGE patterns when digested with SfiⅠ.The predominant patterns in two outbreaks were the same.The other most breaks had different predominant patterns.The PFGE patterns of isolates from the environment were inconsistent.It's indicated that the sources of infection causing these breaks were complicated.PFGE is a discriminatory and reproducible strain typing technique in epidemiologic analysis of microorganisms.