Neuroprotection effect of FLOS ROSAE RUGOSAE extracts on PC12 cells against Aβ25-35-induced cytotoxity.

[Objective]To study the neuroprotection effect of FLOS ROSAE RUGOSAE on PC12 cells against Aβ25-35 -induced cytotoxity. [Method] After being co-incubated with Aβ25 -35 ,the viability of PC12 cells was determined by MTT method,content of MDA was determined by colorimetry, and the expression level of Nf-κb was evaluated by ELISIA. [Result]The extract of FLOS ROSAE RUGOSAE did not show apparent cytotoxity towards PC12 cells; instead,it inhibited the cytotoxity induced by Aβ25-35 . Compared with the model group,the content of MDA and expression of Nf-κb in PC12 cell were decreased after being treated with the extract of FLOS ROSAE RUGOSAE. [Conclusion]The extracts of FLOS ROSAE RUG-OSAE showed neuroprotection effect on the cytotoxity of PC12 induced by Aβ25-35 ,whose mechanism may be related to the decreasing content of MDA and Nf-κb in PC12 cells caused by Aβ-induced oxidative stress.