Interaction Mechanism between Bromocresol Green and Bovine Serum Albumin

The mechanism of color changes of Bro mocresol Green(BCG)and Bovine Serum Albumin(BSA)in acid condition was studied by spectrometry.The influence of differen t experimental conditions on the abso rption spectra of BCG -BSA complex wa s investigated and some reasonable explanations were d eveloped.It is found that color chan ges of the BCG -BSA complex mainly arise from the hydrop hobic interaction between BCG and BSA.