Deletion mutantion chiB promoter, and application of constitutive expression protein
2012
One deletion mutation in a gene encoding a chitinase promoter and its application chiB constitutive expression of the protein. First, the present invention provides a DNA molecule directed breeding, after deletion of the DNA obtained by the method to operate a mutated gene encoding a chitinase promoter sequence chiB --chiBp △ 135, the sequence list SEQIDNo.1 shows. The promoter sequence, a nucleotide sequence of the gene can be made thereto connected constitutively expressed. This mutation can be used in a promoter Bacillus thuringiensis or E. coli cells constitutively, expression of large proteins.
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