Detection of Salmonella Group with Monoclonal Antibodies

열처리된 Salmonella typhimurium 세포를 이용하여 Salmonella에 대한 단일클론성 항체를 생산하는 hybridoma 3종을 얻었다. 3종의 hybridoma는 각각 다른 항체 IgG1 k, IgG2a k, IgM을 생산하였으며 이들 항체는 EIA로 Salmonella와 다른 세균들에 대한 특이성을 검토하였다. IgG1 k 항체는 시험된 Salmonella균과 Enterobacter clocae 및 몇종의 E. coli strain과 친화성이 있었으며 IgM항체는 대부분의 세균에 대해서 친화성을 나타내었다. IgG2a k 항체는 Salmonella strain과 E. coli strain B에 약한 친화성을 나타내었다. IgG2a k 항체는 modified double antibody sandwich EIA에서 Salmonella 항원을 $5{\times}10^3cells/ml$ 농도까지 검출 가능하였으며 항체역가는 mouse 복수액에서 $5{\times}10^4$ 이었다. 【Monoclonal antibodies were produced against whole cells of heat treated Salmonella typhimurium. From the Balb/c mice immunized, 164 hybridomas were obtained. Of 164 hybridomas, six reacted with all three species of Salmonella; three were cloned. Monoclonal antibodies from three hybridomas were characterized to belong to IgG1 k, IgG2a k, IgM subclass respectively. Among them, IgG2a k revealed the most specific reactivity of Salmonella species tested, even though reacted weakly with one of E. coli strains, whereas IgGl k and IgM were found to cross react with some strains of Enterobacteriaceae. By using a modified double antibody sandwich EIA together with IgG2a k, a minimum level of cells of Salmonella species detected was estimated $5{\times}10^3$ cells/ml. Antibody titer of IgG2a k in ascite fluids was $5{\times}10^4$ .】