УХУДШЕНИЕ ЖИЗНЕСПОСОБНОСТИ ТРАНСФОРМИРОВАННЫХ КЛЕТОК КИТАЙСКОГО ХОМЯЧКА В НЕПЕРЕСЕВАЕМОЙ КУЛЬТУРЕ ПОД ВЛИЯНИЕМ ЭКЗОГЕННОГО ОКИСЛЕННОГО ГУАНОЗИДА ПРОЯВЛЯЕТСЯ ТОЛЬКО В СТАЦИОНАРНОЙ ФАЗЕ РОСТА

Despite the fact that oxidation products of nucleotides and nucleosides are markers of oxidative stress, reports of the paradoxical ability of these compounds to protect cells from the harmful effects of reactive oxygen species began to appear more often. Among all nitrogenous bases, guanine is most susceptible to the influence of oxidative stress, therefore, guanosine is oxidized more often than other bases. In the present work, the effect of exogenous 8-oxo-2'-deoxyguanosine on the growth and “stationary phase aging” (accumulation of “age” changes in cultured cells during cell proliferation slowing down within a single passage and subsequent “aging” in the stationary phase of growth) of a nonsubcultured culture of transformed Chinese hamster cells was studied. We showed that the nucleoside is rapidly absorbed by the cells from the medium, but it does not affect the growth of the culture, and in the late stationary phase of growth impairs the viability of the cells. Thus, no mitogenic or geroprotective effect of 8-oxo-2'-deoxyguanosine was found.