Evaluation of Human Serum of Severe Rheumatoid Arthritis by Confocal Raman Spectroscopy
2010
Rheumatoid Arthritis is a systemic chronic inflammatory disease, recurrent and systemic, initiated by
autoantibodies and maintained by inflammatory mechanisms cellular applicants. The evaluation of this
disease to promote early diagnosis, need an associations of many tools, such as clinical, physical
examination and thorough medical history. However, there is no satisfactory consensus due to its
complexity. In the present work, confocal Raman spectroscopy was used to evaluate the biochemical
composition of human serum of 40 volunteers, 24 patients with rheumatoid arthritis presenting clinical
signs and symptoms, and 16 healthy donors. The technique of latex agglutination for the polystyrene
covered with human immunoglobulin G and PCR (protein c-reactive) was performed for confirmation
of possible false-negative results within the groups, facilitating the statistical interpretation and
validation of the technique. This study aimed to verify the changes for the characteristics Raman peaks
of biomolecules such as immunoglobulins amides and protein. The results were highly significant with a
good separation between groups mentioned. The discriminant analysis was performed through the
principal components and correctly identified 92% of the donors. Based on these results, we observed
the behavior of arthritis autoimmune, evident in certain spectral regions that characterize the
serological differences between the groups.
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