Oral Candida Genotyping by PCR ITS1-ITS2 and RAPD

Objective To genotype 9 candida Clinical isolates by PCR ITS1-ITS2 and RAPD methods, and compare the results of these two methods. Methods Nine Candida species identified by YBC Test Kit included 7 C.albicans,1 C.tropicalias and 1 C.glabrates. PCR ITS1-ITS2 and RAPD methods were used to genotype 9 Candida species which were isolated from the oral cavity. Results Seven C.albicans were divided into 2 genotypes by PCR ITS1-ITS2,and 2 Candida clinical isolates were identified as 1 C.tropicalias and 1 C.glabrates. Seven C.albicans were divided into 6 genotypes by RAPD,two isolates speculated as non- C.albicans. Conclusion The results of the genotypes between the two methods were not consistent completely. The results suggest that the PCR ITS1-ITS2 method is useful to genotype candida.