Regulation of the Lyophilized Powder of Earthworm on Cholesterol 7α-Hydroxylase Gene in Hyperlipidemic Mice

Kunming mice were fed with high-level lipid feeds to establish the hyperlipidemic mouse model.The mice were treated with the lyophilized powder of earthworm at dosages of 0.4,0.2 and 0.1 g/kg,qd and were fed with high-level lipid feeds for 10 weeks.The total RNA of liver was extracted and the changes of liver cholesterol 7α-hydroxylase(CYP7α1) gene expression were analyzed by RT-PCR.The results indicated that the lyophilized powder of earthworm could significantly up-regulated CYP7α1 mRNA transcription.It suggested that the effect of the lyophilized powder of earthworm on reducing serum cholesterol in hyperlipidemia mice was probably involved in up-regulating CYP7α1 mRNA transcription.