D3.3 - Cell Culture Monitoring System Comprising Superoxide Sensors for Tumour Cell Analysis

A sensor for the selective and sensitive measurement of reactive oxygen species (ROS) candidate superoxide was developed using direct electrochemical oxidation of superoxide radical on gold electrodes at low operation potential. Sensor performance regarding sensitivity and selectivity was investigated and optimised by integration of polymer based cut-off membranes. Sensor calibration was done utilising the artificial enzymatic based production of short-lived superoxide radicals by xanthine/xanthine oxidase system. The reaction kinetics of superoxide production was investigated with electrochemical, optical and magneto-spectroscopic measurement methods. Sensor design and fabrication process allow a full integration of superoxide sensor in existing multiparameter measurement platform SCCF for in vitro cell culture monitoring.