Establishment and Application of Transcription-Polymerase Chain Reaction for Identification of Field Isolate of Porcine Pseudorabies Virus and Vaccine Strain

On the base of gE and gB gene design two primers to distinguish vaccine strains and wild strains.Using this PCR method to detect PRV,PCV2,PPV,PEDV,PRRSV and only PRV could be detected.Using this method to make etiology survey in Jilin province,32 cases were detected as pseudorabies rabies virus infection in 205 cases,the positive rate was 15.6%.