Synthesis of Phospholipids and Triglycerides in Human Liver Slices III. Influence of Bile Acids, Choline, and Linoleic Acid

Human liver slices were incubated with labelled glycerol and the incorporation of label into triglycerides and different phosphoglycerides was determined. Replacement of palmitic acid by linoleic acid in the incubation medium resulted in a decrease of the glycerol incorporation rate into phosphoglycerides. In response to addition of bile acids or choline to the incubation medium, a change in the distribution of incorporated glycerol among the different phosphoglyceride classes was observed. The incorporation rate into ethanol amine-phosphoglyceride was decreased and into choline phosphoglyceride increased. This finding indicates that bile acids exert a direct effect on the synthesis of phosphoglycerides in the liver.