Construction and expression of Porcine Cecropin P1 expression vector

Three gene fragments were designed and synthesized according to the preference of E.coli.The gene of Cecropin P1 was amplified by PCR and cloned into prokaryotic expression vector pMAL-p2X.The recombinant expression plasmid pMAL-p2X-cecropin P1 was transformed into E.coli TB1.The fusion protein MBPCecropin P1 was produced by IPTG induction and purified by Amylose Resin affinity collumn.The analysis of SDS-PAGE showed that the MBPCecropin P1 fusion protein was expressed and purified.The expression of Cecropin P1 gene may lay a foundation on research of antimicrobia1 activities and its mechanism.