Real-time calcium imaging by the interaction of adenosine receptors in living HEK293T cells

Adenosine aggravates pain via the interactions of adenosine A2A receptor (A2AR) and adenosine A1 receptor (A1R). And heteromerization of A2AR and A1R can put adenosine into operation. Simultaneously, a growing body of information indicates Ca2+ participate in pain management. Our team proved the interaction between A1R and A2AR by Iem-spFRET method. In the present study, we estimated the influence of the dimerization on the concentration of intracellular Ca2+. These dates demonstrate that the interaction of pain-related receptors (A2AR and A1R) has a significant effect on intracellular Ca2+ signaling.