Effect of procaine on membrane potential and intracellular pH in Xenopus laevis oocytes

The effect of the local anaesthetic procaine on the intracellular pH, pHi, and electrophysiological properties of full-grown Xenopus oocytes was studied. In spite of its interference with both the pH-sensitive microelectrodes and fluorescent probe BCECF, we have shown that procaine induced an intracellular acidification rather than the alkalization commonly observed in most cells. The resting pHi of Xenopus oocytes loaded with BCECF was 7.36 ± 0.04 (n = 16). Addition of 10 mM procaine to the bath at pH 7.5 caused pHi to decrease to a new steady state value of 6.97 ± 0.05 (n = 9). A similar behaviour of pHi was observed with microelectrodes. Procaine also promoted a rise in membrane conductance and a membrane depolarization. These changes in membrane potential and conductance were not caused by the decrease in pHi since the addition of sodium propionate at pH 7.5 produced the same decrease of pHi as procaine, but resulted in only a slight depolarization with superimposed oscillations. Current measurements ...