Monolayer culturing and cloning of human pluripotent stem cells on laminin-521–based matrices under xeno-free and chemically defined conditions

Sergey Rodin,Liselotte Antonsson,Outi Hovatta,Karl Tryggvason

Monolayer culturing and cloning of human pluripotent stem cells on laminin-521–based matrices under xeno-free and chemically defined conditions

2014

Sergey Rodin
Liselotte Antonsson
Outi Hovatta
Karl Tryggvason

Coating of cell culture plates with laminin-521 allows culturing of human pluripotent stem cells and, in combination with E-cadherin, their cloning in individual wells.

Keywords:

Laminin
Cell biology
Induced pluripotent stem cell
Cloning
Monolayer
Molecular biology
Biology
Cell culture
Cellular differentiation
xeno free

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