GeLC-MS-based proteomics of Chromobacterium violaceum : comparison of proteome changes elicited by hydrogen peroxide
2016
Chromobacterium violaceum is a free-living bacillus with several genes that enables it survival under
different harsh environments such as oxidative and temperature stresses. Here we performed a labelfree
quantitative proteomic study to unravel the molecular mechanisms that enable C. violaceum to
survive oxidative stress. To achieve this, total proteins extracted from control and C. violaceum cultures
exposed during two hours with 8mM hydrogen peroxide were analyzed using GeLC-MS proteomics.
Analysis revealed that under the stress condition, the bacterium expressed proteins that protected
it from the damage caused by reactive oxygen condition and decreasing the abundance of proteins
responsible for bacterial growth and catabolism. GeLC-MS proteomics analysis provided an overview
of the metabolic pathways involved in the response of C. violaceum to oxidative stress ultimately
aggregating knowledge of the response of this organism to environmental stress. This study identified
approximately 1500 proteins, generating the largest proteomic coverage of C. violaceum so far. We also
detected proteins with unknown function that we hypothesize to be part of new mechanisms related to
oxidative stress defense. Finally, we identified the mechanism of clustered regularly interspaced short
palindromic repeats (CRISPR), which has not yet been reported for this organism.
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