Study of the system of tuberous root induction in vitro from Rehmannia glutinosa

This study investigated the induction system of tuberous root in vitro from Rehmannia glutinosa . The roles of plant growth substance, carbohydrates, and minerals were evaluated for induction and development of tuberous root in vitro . The results show that Murashige and Skoog (MS) contributed greatly to induction of tuberous root in vitro , followed by α-naphthalene acetic acid (NAA), sucrose and 6-benzyladenine (BA). The optimal medium was ¼ MS supplemented with 1.5 mgL -1 BA, 0.15 mgL -1 NAA and 5% sucrose. In addition, paclobutrazol (PP333) and methyl jasmonate (MeJA) also played an important role in induction of tuberous root in vitro, the most appropriate concentrations were 1 mgL -1 and 10 μmolL -1 respectively. Key words : Rehmannia glutinosa , tuberous root, tissue culture in vitro .