TREATMENT OF ACUTE SOFT TISSUE INJURIES DURING SUBACUTE STAGE: AN EXPERIMENTAL STUDY

Objective To observe the therapeutic result of acute soft tissue injuries treated during subacute stage in rat,and explore its possible mechanism. Methods Sixty newly born Wistar rats were evenly randomized to control group,cold treatment group,thermotherapy group and alternate-cold-and-heat group.A rat model of acute soft tissue injury at subacute stage was created.A corresponding therapy was then offered to the rats in each group.All the rats were sacrificed 72 hours later.Muscle tissue of affected part was taken and HE stain done for histology.The expressions of TGF-β1,superoxide dismutase(SOD),and malondialdehyde(MDA) were detected using immunohistochemistry. Results Compared with the control,the histological grading was lower in the thermotherapy group and alternate group,the difference being significant(F=37.30;q=7.42,13.36;P0.05);the expressions of TGF-β1 and SOD were higher,and that of MDA was lower(F=10.40-34.77;q=2.14-59.58;P0.05).Compared with the thermotherapy group,the expressions of TGF-β1 and SOD in the alternate group were higher and that of MDA was lower(q=2.05-5.89,P0.05). Conclusion Thermotherapy and alternate-cold-and-heat therapy are proved to be effective for acute soft tissue injuries during subacute stage treatment,the mechanism is likely by raising the expressions of TGF-β1 and SOD,and lowering that of MDA,the alternate cold and heat being superior to the thermotherapy.