In vitro bio-accessibility and distribution characteristic of each arsenic species in different fishes and shellfishes/shrimps collected from Fujian of China.

It is very important to consider the bio-accessibilities and concentrations of each arsenic species, not total arsenic, in seafood in order to accurately assess internal exposure level and health risk of arsenic from seafood. Herein, the concentrations and in vitro bio-accessibilities of each arsenic species in various fishes and shellfishes/shrimps were extensively investigated. Experimental results showed that arsenic species and contents in shellfishes/fishes remarkably varied with the difference of fish/shellfish species or individuals and sampling area, and arsenobetaine (AsB) is dominant arsenic species for fishes and shellfishes/shrimps. Different arsenic species in the same fish/shellfish have quite different bio-accessibilities, and the bio-accessibilities of each arsenic species also varied with fish/shellfish species or individuals. As3+ in fishes/shellfishes was partly oxidized to form As5+ during gastrointestinal digestion, and thus it is more reasonable and practicable to evaluate the bio-accessibilities of inorganic arsenic (iAs, total As3+ and As5+), not individual As3+ and As5+. Fishes and shellfishes/shrimps have similar bio-accessibilities of iAs, AsB and total arsenic, whereas have different bio-accessibilities of MMA (monomethylarsonic acid), DMA (dimethylarsinic acid), and two un-identified arsenic (Ui-As1 and Ui-As2). The results of this study provided a valuable knowledge for accurately assessing the health risk of arsenic in seafood.