Automation of a Multiplexed Competitive Luminex Immunoassay for Measuring Antibodies to Human Papillomavirus Types 6, 11, 16, and 18

Serum specimen pipetting is a labor-intensive process that can be a source of laboratory error, ergonomic stress, and a potential source of accidental exposure to infectious pathogens. Automated solutions for sample preparation reduce time spent on sample handling, minimize exposure to infectious pathogens, reduce laboratory error, reduce ergonomic stress, and save money. A fully automated method was developed for a custom-designed competitive Luminex immunoassay (cLIA) on a TECAN Genesis Workstation that measures antibodies to human papillomavirus (HPV) virus-like particles (VLPs). The automated program generated statistically similar data to assays performed manually. Overall, HPV 6, 11, 16, and 18 antibody titers obtained from samples prepared with the automated program were 3.2%, 6.6%, 3.6%, and 2.0% higher, respectively, than antibody titers from samples prepared manually. The agreement rates between methods for HPV positive and negative samples for HPV 6, 11, 16, and 18 were 100%, 96.8%, 98.4%, and ...