TheIndirect Association ofHumanT-Cell LeukemiaVirus tax Protein withDNA Results inTranscriptional Activation

that hasnoenhancersequence. ThepCGTAXwasconstructedbyinsertingthecomplete taxcoding sequenceinto pCGvector(33) that has the cytomegalovirus promoter. Cells weretransfected withnoactivatorplasmid(lane 1), pCGTAX(lane 2), orGAL4-TAX(lane 3). (C) Thetax expression plasmid pCGTAX(0.1 ,ug) orits mutants (1.0 ,ug) and2.0 jig ofthe reporter plasmid 21WT-CAT (6), which contains 21-bp repeats, were cotransfected. For lane 7, 0.1 ,ug of d338/353(end) was used.Descriptions oflanes are as for panel A, except that GAL4-TAXconstructs were replaced bythe tax construct pCGTAX.