Dry matter intake, performance, carcass traits and expression of genes of muscle protein metabolism in cattle fed increasing levels of de-oiled wet distillers grains: Use of wet distillers grains in feedlot cattle diet

Abstract The supply of wet distillers grains (WDG) has been growing in Brazil, with the increase in corn ethanol production. Thus, the aim of this study was to evaluate the effect of de-oiled corn WDG in the diet of feedlot cattle on DMI, performance, carcass traits, meat quality and expression of genes involved in the muscle protein metabolism. One hundred non-castrated F1 Angus-Nellore bulls, aged 20–24 months, with average IBW of 369.58 ± 49.17 kg, were used in a randomized complete block design. The animals were fed diets containing 0, 150, 300 and 450 g/kg de-oiled WDG (4% EE) replacing corn and soybean meal. After 129 days on feed, the animals were slaughtered and LT muscle samples were collected for molecular biology assays. Muscle protein metabolism genes such as eIF4, IGFR1, mTOR, GSK3β and P70S6K were investigated by RTq-PCR. Data were analyzed using polynomial contrasts (linear, quadratic and control versus WDG). The inclusion of by-product improved DMI and FBW (P  0.05) among treatments (SF, CL, ILC, MFI, and WHC). However, the inclusion of WDG led to a reduction in the expression of eIF4, mTOR and IGFR1 genes (P