[Study on apoptosis effect induced by isothiocyanates in broccoli on HepG-2 cells and its mechanism].

Objective: To investigate the apoptosis effect of isothiocyanates(ITCS) on human liver cancer cells HepG-2,and its mechanism.Method: HepG-2 cells were treated with different concentrations of ITCS.MTT assay was used to evaluate the influence of ITCS on cell proliferation.Flow cytometry was used to test ROS levels,intracellular mitochondrial transmembrane potential(Δψm),and hypodiploid apoptosis peak in HepG-2 cells.Result: ITCS obviously inhibited proliferation of HepG-2 cells.When treated with 15,30,60,120,240 μg·mL-1 of ITCS for 24 h,ROS levels were(23.1±1.8)%,(53.3±3.3)%,(57.9±2.0)%,(79.9±3.4)%,(93.4±2.6)% respectively;and Δψm were(94.8±5.5)%,(91.8±5.4)%,(66.0±5.6)%,(65.5±6.6)% and(44.3±2.7)% respectively;when treated with 60,120,240 μg·mL-1 of ITCS for 48 h,cell apoptotic rates were(16.6±2.8)%,(21.9±4.4)% and(70.2±5.3)% respectively.Conclusion: ITCS generates ROS in gastric cancer HepG-2 cells,which causes mitochondrial membrane permeabilization and Δψm decrease,therefore, leads to apoptosis of HepG-2 cells.