Analysis of CYP2A6 and CYP2A13 genes using new generation sequencing method in smokers and non-smokers

Aims: CYP2A6 and CYP2A13 genes are sub grouped in CYP2A gene cluster and have important role in nicotine detoxification mechanisms. We aimed to determine the variants of CYP2A6 and CYP2A13 in smokers and non-smokers. Material and Methods: 64 smokers and 36 non-smokers were included in the study. DNAs were isolated from 100 peripheral blood samples. The amplicants of genes using the primers which were designed by “Primer-BLAST” program were sequenced in “Illimuna-MiseqQ-platform. Results: Mean age was 47±13 for smokers, and 34±12 for non-smokers. The age and gender difference between smokers and non-smokers was significant (p Conclusion: The full genome sequence in CYP2A6 and CYP2A13 genes analysed for the first time in our study. CYP2A6 gene variants distrubition and numbers with the different haplotypes were significantly increased (5 and 6 in smokers, 3 and 10 in non-smokers) . CYP2A13 gene variants were increased in smokers compared to non-smokers. The result supports that the variant haplotypes could be effective in maintaining the smoking addiction. This study was supported by Istanbul Univesity, BAP-ONAP support program .