Isolasi dan Karakterisasi Enzim Karbohidrase Cairan Rumen Sapi Asal Rumah Potong Hewan Isolation and Characterization of Carbohydrases in Beef CaĴ le Rumen Liquor from AbaĴ oir

The aims of this experiment were to identify carbohydrases in rumen liquor of caĴ le, and to determine optimum temperature and pH of enzymes, as well as to evaluate the eff ect of metal ions and chemical substances on enzymes activity. Optimum precipitation of the enzymes from local and imported caĴ le was reached at the concentration of 60% and 70% of ammonium sulphate, respectively. The enzymes (xylanase, mannanase, and amylase) had optimum temperature in the range of 50-60 °C, except for cellulase from imported caĴ le which had optimum temperature at 39 o C, optimum pH of the enzymes were 6-7 except for cellulase was at pH 4. The enzymes mostly needed methal ions as activators. EDTA and β-mercaptoethanol inhibited activity of cellulase from rumen liquor of local caĴ le, while enzymes from rumen liquor of imported caĴ le EDTA inhibited xylanase activity and β- mercaptoethanol inhibited mannanase and amylase activity. Activity of cellulase in rumen liquor of local caĴ le was higher than those of imported caĴ le. It is concluded that rumen liquor of imported and local caĴ les from abbatoir contained cellulase, xylanase, mannase and amylase that most of them had optimum temperature and pH at 50-60 o C and 6-7, respectively, and they needed methal ions as