Cultivation method of tetraploid Anoectochilus roxburghii

The invention provides a cultivation method of tetraploid Anoectochilus roxburghii. The cultivation method comprises such stages as artificial pollination of the Anoectochilus roxburghii, asymbiotic germination, treatment by use of a mutagenic agent, strong seedling culture and test-tube seedling transplanting. After the Anoectochilus roxburghii blooms, the artificial pollination is carried out; the mature capsules of the Anoectochilus roxburghii are sterilized and then cut open to take out embryos, and then the embryos are inoculated to a germination culture medium for cultivation to form protocorms; next, the protocorms are put in a liquid differential medium containing the mutagenic agent to be mutagenized, and then inoculated to a strong seedling culture medium for strong seedling culture, and finally, test-tube seedling transplanting is carried out. The cultivation method is simple and feasible; the mutagenic frequency is above 75%, and meanwhile, the yield of the Anoectochilus roxburghii used as a medicinal material also can be greatly improved; as a result, a new way is opened for the polyploidy breeding, genetic improvement and new variety breeding of the Anoectochilus roxburghii.