Chick small intestine brush border contains lipase and phospholipase activity

1. Uptake of triglycerides and phosphoglycerides was demonstrated in ligated washed chick intestinal segments in situ . Mucosal uptake was observed both in the jejunum and ileum of chicks and was not accompanied by significant luminal hydrolysis of acyglycerides. 2. Brush border membrane vesicles were prepared and lipase and phospholipase activities were demonstrated. These activities were enriched in parallel to the increase in activity of maltase and gamma-glutamyl transpeptidase in all small intestinal areas. 3. In order to determine if the pancreatic and brush border membrane lipolytic activities were similar different properties of these activities were examined. The relative activities of pancreatic and brush border membranes towards triolein and phosphatidylcholine differed. The pH-triolein activity curve and the use of different additives showed some diverging effects between the activities. These differences were not, however, sufficient to conclude that the brush border activity towards triolein...