Phyllobacterium salinisoli sp. nov., isolated from a Lotus lancerottensis root nodule in saline soil from Lanzarote
2018
A Gram-negative rod, designated strain LLAN61T, was isolated from a root nodule of Lotus lancerottensis growing in a saline soil sample from Lanzarote (Canary Islands). The strain grew optimally at 0.5 % (w/v) NaCl and tolerated up to 3.5 %. The 16S rRNA gene sequence analysis showed that strain LLAN61T belonged to genus
Phyllobacterium
and that
Phyllobacterium
leguminum
ORS 1419T and
Phyllobacterium
myrsinacearum
IAM 13584T are the closest related species with 97.93 and 97.86% similarity values, respectively. In the atpD phylogeny,
P. leguminum
ORS 1419T and
P. myrsinacearum
ATCC 43591T, sharing similarities of 87.6 and 85.8% respectively, were also the closest species to strain LLAN61T. DNA–DNA hybridization showed an average value of 21 % between strain LLAN61T and
P. leguminum
LMG 22833T, and 6 % with
P. myrsinacearum
ATCC 43590T. The predominant fatty acids were C19 : 0 cyclo ω8c and C18 : 1ω6c/C18 : 1ω7c (summed feature 8). The DNA G+C content was 58.0 mol%. Strain LLAN61T differed from its closest relatives in some culture conditions and in assimilation of several carbon sources. Based upon the results of phylogeny, DNA–DNA hybridization, phenotypic tests and fatty acid analysis, this strain should be classified as a novel species of
Phyllobacterium
for which the name Phyllobacterium salinisoli sp. nov. is proposed (type strain LLAN61T=LMG 30173T = CECT 9417T).
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